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pegfp c1 aβ 1 42 plasmid  (Addgene inc)


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    Addgene inc pegfp c1 aβ 1 42 plasmid
    Pegfp C1 Aβ 1 42 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+a%CE%B2+1+42+plasmid/pMYC-GFP+(Plasmid+%2342142)/pmc10698508-394-1-8
    Average 91 stars, based on 4 article reviews
    pegfp c1 aβ 1 42 plasmid - by Bioz Stars, 2026-09
    91/100 stars

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    Plasmid Preparation:

    Article Title: A multi‐layered network model identifies Akt1 as a common modulator of neurodegeneration
    Article Snippet: HEK293 cells having 70–80% cell density were transiently transfected with pEGFP‐C1‐Aβ 1‐42 , pEGFP‐Htt‐exon1‐Q74 (Addgene #40262), pEGFP‐Ataxin1‐52Q (Addgene #32492), or pEGFP‐C1‐Ataxin3‐Q84 (Addgene #22123) DNA using Lipofectamine 2000 (Invitrogen, CA, USA) following the manufacturer's instructions. .. The pEGFP‐C1‐Aβ 1–42 plasmid was constructed from pCAX‐FLAG‐APP (Addgene #30154). .. Before drug treatment, HEK293 cells were washed with treating medium (Minimum essential medium (MEM, Gibco, MD, USA) with 1% FBS) and then treated with the indicated concentration of SC79 (0.1–100 μM) (SML0749, Sigma‐Aldrich) for 24 h. Cell death was measured using Cell Counting Kit‐8 (CK04, Dojindo, MD, USA), which was performed according to the manufacturer's instructions.

    Construct:

    Article Title: A multi‐layered network model identifies Akt1 as a common modulator of neurodegeneration
    Article Snippet: HEK293 cells having 70–80% cell density were transiently transfected with pEGFP‐C1‐Aβ 1‐42 , pEGFP‐Htt‐exon1‐Q74 (Addgene #40262), pEGFP‐Ataxin1‐52Q (Addgene #32492), or pEGFP‐C1‐Ataxin3‐Q84 (Addgene #22123) DNA using Lipofectamine 2000 (Invitrogen, CA, USA) following the manufacturer's instructions. .. The pEGFP‐C1‐Aβ 1–42 plasmid was constructed from pCAX‐FLAG‐APP (Addgene #30154). .. Before drug treatment, HEK293 cells were washed with treating medium (Minimum essential medium (MEM, Gibco, MD, USA) with 1% FBS) and then treated with the indicated concentration of SC79 (0.1–100 μM) (SML0749, Sigma‐Aldrich) for 24 h. Cell death was measured using Cell Counting Kit‐8 (CK04, Dojindo, MD, USA), which was performed according to the manufacturer's instructions.



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    Addgene inc pegfp c1 aβ 1 42 plasmid
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